ABSTRACT
Introdução: O sucesso de um enxerto de pele é avaliado não apenas pela integração do enxerto em si, mas também pela qualidade da recuperação da área doadora. A despeito de as áreas doadoras de enxertos representarem o melhor local para estudo de cicatrização de feridas, regimes de tratamento, de áreas doadoras, tem sido incompletamente estudados. Objetivo: Avaliação da eficácia de espumas de poliuretano como curativo de áreas doadoras de enxertos. Método: Estudo prospectivo no qual áreas doadoras de enxertos foram tratadas com espumas de poliuretano como alternativa a filmes de acetato de celulose. Resultados: Foram tratados 11 pacientes e catorze áreas doadoras de enxerto. Aderência prolongada (73%) e odor desagradável (45%) foram os problemas encontrados. Os resultados foram considerados insatisfatórios na grande maioria dos casos (73%). Conclusões: O uso de espumas de poliuretano mostrou-se ineficaz, nesse grupo de pacientes, devido à ocorrência de alto índice de complicações.
Introduction: The success of a skin graft is evaluated by not only the integration of the graft itself, but also the quality of the recovery of the donor site. Despite the fact that graft donor sites represent the best place to study wound healing, treatment regimens for donor sites have not been studied extensively. Method: To evaluate the efficiency of polyurethane foam as a dressing for graft donor sites. Methods: We conducted a prospective study in which graft donor sites were treated with polyurethane foam dressing, as an alternative to a cellulose acetate film. Results: We treated 11 patients and 14 donor graft sites. Problems associated with the use of polyurethane foam included prolonged adherence (73%) and an unpleasant odor (45%). The majority of patients reported that they found the dressing to be unsatisfactory (73%). Conclusions: The use of a polyurethane foam was shown to be ineffective as a graft donor site dressing, due to the high rate of associated complications.
Subject(s)
Humans , Male , Female , Adult , Middle Aged , History, 21st Century , Polyurethanes , Postoperative Complications , Skin , Bandages , Wound Healing , Wounds and Injuries , Biological Dressings , Efficacy , Prospective Studies , Skin Transplantation , Evaluation Study , Dermatologic Surgical Procedures , Polyurethanes/analysis , Polyurethanes/therapeutic use , Postoperative Complications/surgery , Skin/anatomy & histology , Bandages/adverse effects , Bandages/standards , Wounds and Injuries/surgery , Wounds and Injuries/therapy , Biological Dressings/adverse effects , Biological Dressings/standards , Efficacy/methods , Skin Transplantation/adverse effects , Skin Transplantation/methods , Skin Transplantation/standards , Dermatologic Surgical Procedures/adverse effects , Dermatologic Surgical Procedures/methodsABSTRACT
Chitosanase production of Gongronella sp. JG cells immobilized in calcium alginate gel and polyurethane foam was compared with that of the free cells, there was a 60% increase in the enzyme yield (2429 U/L) compared to the highest yield obtained from free cells (1513 U/L). The optimal immobilization parameters (concentrations of sodium alginate, calcium chloride, bead inoculums, bead diameter, etc) for the enhanced production of chitosanase were determined as: sodium alginate 2% (w/v), 0.1 M calcium chloride, inoculum 10 mL beads to 100 mL production media and 2.7 mm bead diameter. Maximum chitosanase production was achieved with initial pH of 5.5 and temperature of 30 ºC. The alginate beads had well stability, retained 85% ability of enzyme production even after 7 cycles of repeated batch fermentation. These results showed the immobilization technique was a feasible and economical method for chitosansase production by Gongronella sp. JG.
Subject(s)
Animals , Alginates , Crustacea/enzymology , Crustacea/microbiology , Fermentation , Aquatic Fungi/analysis , Polyurethanes/analysis , Chitosan/analysis , Chitosan/isolation & purification , Sodium/analysis , Attention , Cells, Immobilized , Enzyme Activation , Food Samples , Methods , Reference StandardsABSTRACT
Estudo da neoformaçäo óssea basal da mandíbula de 60 ratos, após preparo de um defeito obtido com fresa cilíndro-cônica, e preenchimento do leito ósseo de 30 ratos do grupo experimental com poliuretana vegetal acrescida de CaCO3. Para os 30 animais do grupo controle, o defeito ósseo näo recebeu nenhum tratamento. Aos 15, 25, 40, 60, 090 e 120 dias após a cirurgia, os animais foram sacrificados e suas mandíbulas removidas e coradas com hematoxilina e eosina, Picrosírius e tricrômico de Mallory para o estudo morfológico sob microscopia óptica comum e de polarizaçäo. Os resultados demonstram grande aceitaçäo orgânica à resina, näo ocorrendo formaçäo de cápsula, tampouco migraçäo local de células inflamatórias, além de o carbonato funcionar como isca, promovendo neoformaçäo óssea, estimulando o aparecimento das células osteogênicas